Things used in this project

Hardware components:
peristaltic pump
×1
Fairchild semiconductor pn2222abu. image
General Purpose Transistor NPN
×1
Fairchild semiconductor 1n4004. image
1N4007 – High Voltage, High Current Rated Diode
×1
phenolphtaleine
×1
Intel.web.720.405
Intel Edison
×1
13036 01
SparkFun Block for Intel® Edison - Arduino
×1
Camera (generic)
×1
Tens70
9V battery (generic)
×2
Software apps and online services:
Q8wtlimqnp04fzhtr9v5
IFTTT Maker service
73318 301258139977848 644841747 n
OpenCV
Hand tools and fabrication machines:
Form Labs 3D printer

Custom parts and enclosures

CAD files for microfluidic prototype
Print on Form2 with translucent resin
F7Eg4UfPx8GbMVHUbHfS.3dm

Schematics

Render of millifluidic device
plug it in
5aalg8poqe44qugc668i

Code

DNA detectorPython
This script runs the pump, camera, processes images with openCV and sends a text message with results via IFTTT
#!/usr/bin/env python

import mraa
import time
import numpy as np 
import cv2
import os


button = mraa.Gpio(6)
button.dir(mraa.DIR_IN)

pump = mraa.Gpio(11)
pump.dir(mraa.DIR_OUT)
pump.write(0)

led = mraa.Gpio(12)
led.dir(mraa.DIR_OUT)
led.write(0)

buzzer = mraa.Gpio(4)
buzzer.dir(mraa.DIR_OUT)
buzzer.write(0)

cap = cv2.VideoCapture(0)
ret, frame = cap.read()

# delays
pumpDelay = 45
soakDelay = 15

# iteration = 0


# define range of pink color in HSV
lower_pink = np.array([110,50,50])
upper_pink = np.array([130,255,255])


def captureImage():
        # Take each frame
        ret, frame = cap.read()
        print "read ret: " + str(ret)

        # Convert BGR to HSV
        hsv = cv2.cvtColor(frame, cv2.COLOR_BGR2HSV)
        return hsv

def readImage(imgname):
        print "read image" + imgname
        img = cv2.imread(imgname,0)
        # Convert BGR to HSV
        hsv = cv2.cvtColor(img, cv2.COLOR_RGB2HSV)
        return hsv


def calculatePinkPixels(image):
        mask = cv2.inRange(image, lower_pink, upper_pink)
        return cv2.countNonZero(mask)



def getButtonPress():
        while True:
                if (button.read() == 1):
			print "Button Pressed %f" % (time.time())
                        return
                else:
                        time.sleep(0.05)

def sendCurl(message):
        command = "curl -X POST -H \"Content-Type: application/json\" -d '{\"value1\":\"%s\"}' https://maker.ifttt.com/trigger/dnaDetected/with/key/cTs_Jqc-oa-NLl-jo6dKL1" % (message)
        os.system(command)

def chirpBuzz():
	buzzer.write(1)
	time.sleep(0.05)
        buzzer.write(0)

def takeMeasurement(iteration):
        print "...cap img 1"
        img1 = captureImage()
        # img1 = readImage("test_img#1.jpg")
        cv2.imwrite('/home/root/programs/it%d-img1.jpg' % (iteration), img1, [cv2.IMWRITE_JPEG_QUALITY, 90])
        
        print "...pump 1"
        pump.write(1)
        time.sleep(pumpDelay)

        print "...pump 0"
        pump.write(0)

        time.sleep(soakDelay)
        
        print "...cap img 2"
        img2 = captureImage()
        # img1 = readImage("test_img#2.jpg")
        cv2.imwrite('/home/root/programs/it%d-img2.jpg' % (iteration), img2, [cv2.IMWRITE_JPEG_QUALITY, 90])

        print "...calc pixels"
        diffPinkPixels = calculatePinkPixels(img2) - calculatePinkPixels(img1)
        print "...diff pink pixels %d" % (diffPinkPixels)

        if diffPinkPixels > 100:
                message = "DETECTED"
        else:
                message = "UNDETECTED"

        print "...send curl"
        sendCurl(message)

def run():
        iteration = 0
        while True:
                getButtonPress()

                print "buttonPressed"
                led.write(1)
                print "led on"
                print "start measurement"
                takeMeasurement(iteration)
                print "end measurement"
                led.write(0)
                iteration = iteration + 1



def end():
        print "releasing camera"
        cap.release()        
        

try:
        run()
finally:
        end()

Credits

Vexogkqjv6a2idbkyj8a
Léonard Roussel

Acoustic engineer, sound designer. Grew up in Europe, live in NYC, want to do more sound art by adding behavior to physical or artificial objects.

Replications

Did you replicate this project? Share it!

I made one

Love this project? Think it could be improved? Tell us what you think!

Give feedback

Comments

Similar projects you might like

J.A.R.V.I.S. : A Virtual Home Assistant
Advanced
  • 5,618
  • 81

Full instructions

J.A.R.V.I.S. is a personal home automation assistant for controlling electrical home appliances integrated with an augmented reality app.

Biomed Shield for Arduino 101 Powered By Intel
Advanced
  • 5,352
  • 17

Work in progress

Allows students, educators, and hobbyists to learn about biomedicine by monitoring heart rate, temperature, and other physiological metrics.

Caravaggio, a Drawing Machine
Advanced
  • 8,689
  • 59

Caravaggio is a drawing machine able to replicate a digital image using a pen and a continuous line.

Wolf: Search and  Rescue Multi-Terrain Robot
Advanced
  • 2,568
  • 26

Full instructions

Wolf is an IoT multi-terrain robot running on the Arduino 101 platform.

 Traffic Light Information System
Advanced
  • 2,157
  • 15

Full instructions

Traffic Light Information System that lets the driver know at which speed he needs to drive in order to pass the green light.

Animatronic Tail by circuito.io
Advanced
  • 1,757
  • 10

Full instructions

Our first wearable project (!) is an animatronic tail with dual-control. No animals harmed in the making of this project.

ProjectsCommunitiesContestsLiveAppsBetaFree StoreBlogAdd projectSign up / Login
Respect project
Feedback